Caracterização e avaliação in vitro da biocompatibilidade da membrana de PCL incorporada com extrato de Jucá

Abstract

This work aims to characterize and evaluate in vitro the biocompatibility of membranes developed using Polycaprolactone (PCL) and modified by the incorporation of Libidibia ferrea L. (Jucá) extract and chloroform (CHCl3) for dental application. To prepare the samples, the 7.5% dry extract of Jucá was added to the PCL solution. Two groups of membranes were evaluated: PCL + chloroform (control) and PCL + chloroform + Jucá (experimental). The fibers produced for the membrane were synthesized using the rotary spinning method with parameters determined and fixed for all the tests. The incorporation of the Jucá extract into the membrane was tested using Ultraviolet-Visible Spectroscopy (UV-Vis), using samples of the control membrane and Jucá, cut, standardized and weighed, with subsequent extraction of the Jucá from a hydroalcoholic solution and then read in UV-Vis. Micrographs obtained by Scanning Electron Microscopy (SEM) were used to analyze the membrane surfaces, and the morphological characterization of the fibers was obtained. The average diameter of the fibers was obtained and measured using ImageJ software. High performance liquid chromatography (HPLC) identified quercetin as one of the main compounds present in the L. ferrea extract in the 370nm range, quantifying 0.1% quercetin in the extract. Cell biocompatibility was assessed using cytotoxicity and cell migration tests. The cytotoxicity test used was the Alamar blue test, which monitors the reducing environment of the living cell using the resazurin reagent. The results showed that the highest absorbance peak of Jucá in UV-Vis was in the 230nm range. The morphology revealed that the fibers with the addition of 0.1g of Jucá were well formed and randomly distributed. The addition of 0.3g of Jucá extract to the polymer solution altered the diameter of the fibers. The mean diameter (MD) of the control PCL fibers was 22.14 μm ± 9.06 μm, while the MD of the 0.1g PCL+Jucá fibers was 26.72 ± 5.56 μm; and the 0.3g PCL+Jucá membrane showed MD = 9.27 ± 4.11. The PLC+Jucá membrane showed 83.6% viability and 16.3% toxicity, while the pure PCL membrane showed 85% viability and 14.9% toxicity. The in vitro cell migration test showed that PCL membranes loaded with Jucá increased the cell migration process. Thus, based on the results obtained, it is possible to conclude that the membranes have adequate morphological characteristics, favorable cell proliferation and, with this, great potential for use and application as a drug release device in the oral cavity