@MASTERSTHESIS{ 2025:1201345634, title = {Blood physiology of freshwater stingrays from the lower Rio Negro, Amazonas, Brazil}, year = {2025}, url = "https://tede.ufam.edu.br/handle/tede/10958", abstract = "Variations in the physiological characteristics of Potamotrigonines affect hematological parameters, revealing their health status. It is essential to obtain. This study aims to conduct a systematic review of the blood physiology of Potamotrigonines and determine the hematological parameters of freshwater stingrays from the lower Rio Negro, Amazonas. For the systematic review, searches were performed in 3 databases using combinations of keywords, resulting in 368 articles selected according to the inclusion and exclusion criteria. The results showed that only seven Potamotrigonines were studied regarding their blood physiology, with Potamotrygon motoro being the most investigated species. The articles identified analyses of erythrogram, leukogram, thrombogram, plasma biochemistry, hormone levels, enzymes and blood ions. For blood studies of stingrays from the lower Rio Negro, collections were carried out in the Tupé Development Reserve and on Açutuba beach, where 27 specimens were captured, 16 Potamotrygon wallacei, 3 P. motoro and 8 Paratrygon spp. After capture, the animals were anesthetized using an immersion bath, and then blood samples were collected by puncturing the gill vessel for hematological procedures. A multiparametric digital device was used to determine the physicochemical properties of the water. The results of the erythrogram demonstrated hematological differences between the species, as well as the results of plasma biochemistry, which showed high values in cholesterol levels in females, the leukogram and thrombogram demonstrated similar values between P. motoro and Paratrygon sp., while P. wallacei showed lower values for defense cells. The present study contributes with an overview of the physiological state of potamotrigonines and with hematological information on freshwater stingrays from the lower Rio Negro region for the first time. This knowledge is essential to improve care and management protocols for conserving this aquatic resource.", publisher = {Universidade Federal do Amazonas}, scholl = {Programa de Pós-Graduação em Ciência Animal e Recursos Pesqueiros}, note = {Faculdade de Ciências Agrárias} }